I see you are fairly new here. WELCOME to the FORUM! It's good to have you here. I hope you enjoy it as much as the rest of us do... it's a great place!
About your question. Some manufacturers do just cut down shorter lengths of their stock rifle barrels to use for making pistols. That doesn't mean that a 1:66 twist in a 36 inch .50cal rifle barrel is going to work the best in a 9 inch pistol barrel.
Smart manufacturers realize this, and rifle their pistol barrels with a much faster twist--as the velocities are so much lower than in a longer length slow twist rifle barrel. It has to do with Revolutions Per Minute - RPM - rather than what projectile you use in a pistol and only how much velocity is involved..
Still I have seen some pistols with 1:48 twist (same as the companion rifle) that could stand excessively heavy powder charges as the patched round ball wouldn't slip on the rifling and get all wonky on accuracy. Those are exceptions and not typical (at least what I've seen). A reasonably good shooting .45cal or.50cal target pistol might have a 1:18 twist or up to 1:30 twist. While the same caliber rifles might have a 1:48 twist to 1:66 twist. Doesn't matter sometimes at all...
So, YES it "can" make a difference... BUT NO, don't let that stop you from continuing your work. You can always get a faster twist barrel later if you aren't satisfied with the shortened section of rifle barrel.
If you have any extra odds and ends, PM me about them. I might know someone interested in getting a few bits from you.
Shoot Safely!
WV_Hillbilly