I asked my son about his technique, and this is what he said.Alden said:Cool. What did he use for the photomicrograph and what was the (obviosuly overhead) illumination please!?
There are two lenses on a microscope, one right above the specimen, called the objective, the other which you look through called the ocular. This shot had to be taken with the lowest power he has because in microscope terms those powder grains are huge. He used a 4X objective and a 10X ocular for a straight magnification of 40X... then the camera and monitor increase that. You can see the background light under the stage, the round one. I believe the incidence lights he mentions are additional lights he added, which he uses for side and top light, for 3D modeling. The trinocular he speaks of is just a straight tube on top with an adapter to attach the camera, the silver tube in this photo.The final magnification is a little difficult to calculate because of some unknown variables, but I’ve measured it empirically and found that on a 100dpi monitor it’s 75x with that objective. The background light is just the built in light of the microscope turned down real low. It appears brown because it’s a halogen bulb whereas the incidence lights are LEDs, so there’s a color difference. The picture is white balanced for the incidence lighting, making the backlight go brown. My camera is a Canon 20D attached to the trinocular port on the top of the microscope.
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