This is a round ball barrel. What ball diameter are you going to use? Have you measured the bore with calipers? Most of these guns shoot a .490" diameter ball well, but a few do better with a .495" ball. What thickness patching? Most shoot well with .015" patching, but others need thicker patching.
What powder? Real Black Powder or a sub?
What distance are you wanting to zero the gun for?
I am shooting a Green Mountain Barrel with a 1:48" ROT, using .490 balls, .020" patching, Wonder lube, 60 grains of FFg Goex Powder, and have the gun zeroed to shoot 2 inches high at 50 yds. That puts it about 2 inches low at 100 yds with 75 grains of FFg powder in my flintlock. My barrel is 39" long, which is longer than yours. For target work, I use a Walter's Fiber Wad in .510" diameter over the powder to seal gases. I also lube the barrel after seating the PRB.